Magnaporthe oryzae systemic defense trigger 1 (MoSDT1)-mediated metabolites regulate defense response in Rice

Abstract Background Some of the pathogenic effector proteins play an active role in stimulating plant defense system to strengthen resistance. Results In this study, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) was implemented identify altered metabolites transgenic rice containing over-expressed M. oryzae Systemic Defense Trigger 1 (MoSDT1) that infected at three-time points. The characterized dominating were organic acids and their derivatives, oxygen compounds, lipids, lipid-like molecules. Among identified metabolites, shikimate, galactinol, trehalose, D-mannose, linolenic acid, dopamine, tyramine, L-glutamine are precursors for synthesis many secondary Carbohydrate, as well amino acid metabolic, pathways revealed be involved responses resistance strengthening. Conclusion increasing salicylic (SA) jasmonic (JA) content enhanced interactions between JA synthesis/signaling gene, SA synthesis/receptor raffinose/fructose/sucrose synthase cell wall-related genes all contribute response rice. symptoms after infection significantly alleviated when treated with six i.e., galactol, L-glutamine, L-tryptophan, ?-terpinene, dopamine 72 h exogenously. Therefore, these could utilized optimal metabolic marker defense.